Fig. 1

Maximum Likelihood phylogenetic tree reconstructed based on recA-glnII-rpoB concatenated nucleotide sequences, showing the relationships between Bradyrhizobium shewense sp. nov. (in green) and recognized species of the genus Bradyrhizobium as well as the position of type strain B. yuanmingense CCBAU1007 ^T.The tree was constructed by using General Time Reversible model using MEGA version 7. A discrete Gamma distribution was used to model evolutionary rate differences among sites (5 categories (+G, parameter = 0.2999). The rate variation model allowed for some sites to be evolutionarily invariable ([+I], 31.7544% sites). Bootstrap values (100 replicates) are indicated at the branching points. Reference type strains are indicated with superscript ‘T’. Bar, % estimated substitutions. GenBank accession numbers of the sequences (recA, glnII, rpoB in order) are listed in parentheses next to the strains codes. The accession numbers of whole genome sequenced strains are indicated with bold*. Abbreviations: B, Bradyrhizobium ; R, Rhizobium ; sp., species