Fig. 2

Phylogenetic position of the eight sequenced C. boidinii strains based on 18S rRNA sequences. Genbank accession numbers of the aligned sequences are indicated in brackets. C. albicans (strain MUCL29800) 18S rRNA (accession id X53497.1) was used as a query to retrieve the homologues sequences in the other presented species. Sequences were aligned using MUSCLE [37], and the phylogenetic tree was determined using the neighbour-joining algorithm with the Kimura 2-parameter distance model in MEGA (version 7) [38]. A gamma distribution (shape parameter = 1) was used for rate variation among sites. The optimal tree with the sum of branch lengths = 0.1734 is shown, and nodes that appeared in more than 50% of replicate trees in the bootstrap test (1000 replicates) are marked with their bootstrap support values