Fig. 3

In vitro biofilm assay in the selected Elizabethkingia sp. The cells were first cultured by shaking in TSB at 37 °C overnight. The cell density was adjusted to the same OD at 600 nm (0.1). 200 μl of cells were placed on 96-well plates for 24 h. The biofilm assay was carried out using crystal blue staining [49]. Values are mean values for single measurements from eight independent cultures. The error bars are standard deviations. The statistical test was the Student’s t-test. The asterisk indicates a significant difference compared to biofilm formation in E. anophelis Ag1 (p < 0.05).