Permanent draft genome sequence of Frankia sp. NRRL B-16219 reveals the presence of canonical nod genes, which are highly homologous to those detected in Candidatus Frankia Dg1 genome

Table 1 Classification and general features of Frankia sp. strain NRRL B-16219 according to MIGS [45]

MIGS ID	Property	Term	Evidence code^a
	Classification	Domain Bacteria	TAS [46]
		Phylum Actinobacteria	TAS [47]
		Class Actinobacteria	TAS [48]
		Order Frankiales	TAS [49]
		Family Frankiaceae	TAS [50, 51]
		Genus Frankia	TAS [52, 53]
		Species Frankia sp.	IDR
		Strain NRRL B-16219IDA
	Gram stain	Positive	IDA
	Cell shape	Filament-shaped	IDA
	Motility	Non-motile	NAS
	Sporulation	Sporulating	NAS
	Temperature range	25–35 °C	TAS [5]
	Optimum temperature	28 °C	TAS [5]
	pH range; Optimum	pH 6.3 – pH 6.8	NAS
	Carbon source	short fatty acids, TCA-cycle intermediates and carbohydrates	IDA
MIGS-6	Habitat	Soil and Host-associated	IDA
MIGS-6.3	Salinity	Not reported
MIGS-22	Oxygen requirement	Aerobic	NAS
MIGS-15	Biotic relationship	Free-living and Host plant-associated	NAS
MIGS-14	Pathogenicity	Non-pathogen	NAS
MIGS-4	Geographic location	Soil beneath Ceanothus jepsonii, USA	IDA
MIGS-5	Sample collection	1982	IDA
MIGS-4.1	Latitude	Not reported	-
MIGS-4.2	Longitude	Not reported	-
MIGS-4.4	Altitude	Not reported	-

^a Evidence codes – IDA INFERRED FROM DIRECT ASSAY, TAS traceable author statement (i.e., a direct report exists in the literature) NAS non-traceable author statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence)