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Fig. 6 | Standards in Genomic Sciences

Fig. 6

From: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

Fig. 6

Appearance of DNA libraries from Agilent 2100 Bioanalyzer analysis. Representative electropherogram (a) and virtual gel (b) used for visual inspection (generated with the Agilent 2100 Bioanalyzer system with the DNA 12000 Kit) of DNA libraries sizes prepared for sequencing with the PacBio SMRTbell 10 kb Template Preparation Kit on the Agilent NGS Workstation. A typical electropherogram using the Agilent bioanalyzer 2100 DNA 12000 kit shows the lower marker at 35 s and the upper marker at 90 s. The DNA libraries and the upper marker co-elutes with each other, the sharper peak is the upper marker, shown in red on the gel image. The average library sizes are: Campylobacter (green, 9.1 kb), Listeria (blue, 9.5 kb), Vibrio (aqua, 10 kb), and Salmonella (red, 15 kb)

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