Metagenome phylogenetic profiling of microbial community evolution in a tetrachloroethene-contaminated aquifer responding to enhanced reductive dechlorination protocols

Table 10 Geochemical Parameters and Contamination Concentrations

Classification	Parameter	Units	SAE3-0 ^a Mean ± SD	SAE3-5 ^b Mean ± SD	Log₂ Fold-Change
Dissolved Gas by Headspace	Methane	μg/L	BDL^c (<1.0)	6540.0 ± 4292.8	13.7
	Ethene	μg/L	BDL (<2.0)	BDL (<2.0)	0
	Ethane	μg/L	BDL (<2.0)	BDL (<2.0)	0
Anions	Chloride	mg/L	73.7 ± 1.4	83.4 ± 4.7	0.2
	Bromide	mg/L	2.66 ± 2.21	2.48 ± 0.37	−0.1
	Nitrogen, Nitrate (as N)	mg/L	2.05 ± 0.15	BDL (<0.26)	−4.0
	Sulfate	mg/L	213.3 ± 5.2	27.5 ± 30.5	−3.0
Metals	Dissolved Fe	mg/L	0.22 ± 0.08	9.66 ± 9.93	5.5
Metals	Total Mn	mg/L	0.19 ± 0.04	7.54 ± 0.48	5.3
Volatiles	PCE	μg/L	9966.7 ± 1672.9	157.2 ± 196.3	−5.9
	TCE	μg/L	74.7 ± 38.5	223.4 ± 249.6	1.6
	Cis-1,2-DCE	μg/L	390.0 ± 114.7	7840.0 ± 3266.2	4.3
	Trans-1,2-DCE	μg/L	BDL (<125)	143.40 ± 62.90	6.8
	Vinyl chloride	μg/L	BDL (<50)	53.6 ± 21.5	6.7
	Total VOCs	μM	61.248 ± 10.298	85.892 ± 32.489	0.5
TOC	TOC	mg/L	2.13 ± 0.08	104.40 ± 28.95	5.6
Alkalinity	Total (as CaCO₃)	mg/L	311.7 ± 4.1	684.0 ± 89.9	1.1
	Carbonate	mg/L	BDL (<2.0)	BDL (<2.0)	0
	Bicarbonate	mg/L	311.7 ± 4.1	684.0 ± 89.9	1.1
	Hydroxide	mg/L	BDL (<2.0)	BDL (<2.0)	0
	Sulfide	mg/L	BDL (<1.0)	3.28 ± 1.79	2.7
CO₂	Total CO₂	mg/L	291.7 ± 7.5	716.0 ± 103.6	1.3
Field Data	Temperature (°C)	°C	16.723 ± 1.276	20.118 ± 1.844	0.3
	Dissolved O₂	mg/L	0.227 ± 0.166	0.074 ± 0.057	−1.6
	pH		7.250 ± 0.301	7.270 ± 0.334	0.004
	Oxidation-reduction potential		−12.97 ± 46.96	−247.72 ± 44.07	−4.3
	Conductivity	ms/cm3	1.1923 ± 0.1222	1.6318 ± 0.1367	0.5

^aEach value is the average of six geochemical sampling events prior to sampling.
^bEach value is the average of five geochemical sampling events in Nov. 2007, prior to sampling.
^cOne-half of the BDL divided by the dilution factor was used to calculate fold-change. For trans-1,2,-DCE and VC the sample dilution factor was 50. No dilution was used for all other BDL sample results (dilution factor = 1)

ISSN: 2524-6372