Skip to main content

Table 5 Modular PKS and NRPS gene clusters in Micromonospora sp. DSW705

From: Draft genome sequence of Micromonospora sp. DSW705 and distribution of biosynthetic gene clusters for depsipeptides bearing 4-amino-2,4-pentadienoate in actinomycetes

Gene cluster

Encoded in

No. of modular PKS and NRPS genes

No. of modules

Backbone of predicted product

pks/nrps-1 (rak)

scaffold 2

6

7

R-C3-C3 a-Ser-C2-Gly-X

pks/nrps-2

scaffold 2

6

6

X-X-X-?-C2-Ser

pks/nrps-3

scaffold 2

5

6

X-X-?-C2-Asn-Ser

pks-1

scaffold 2

12

33

R-C2-C3-C2-C2-C2-C2-C2-C4-C2-C2-C2-C2-C2-C2-C2-C2-C2-C?-C2-C3-C2-C2-C3-C2-C3-C3-C2-C3-C3-C3-C2-C2

pks-2

scaffold 5

1

1

C2

pks-3

scaffold 24

1

1

C2

nrps-1

scaffold 2

2

2

X-Ala

  1. R starter molecule, C 3 C3 unit derived from methylmalonyl-CoA, C 2 C2 unit derived from malonyl-CoA, X amino acid unpredicted, ? lack of A domain in the NRPS module, C 4 C4 unit derived from ethylmalonyl-CoA or methoxymaronyl-CoA, C ? substrate of AT domain was not predicted
  2. aAlthough antiSMASH predicted that the AT domain incorporates malonyl-CoA as the substrate, the signature sequence for substrate determination is not HAFHS for malonyl-CoA but TSSHS likely for methylmaronyl-CoA [33]