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Table 7 Incorporation of 13C-labeled precursors into rakicidin D

From: Draft genome sequence of Streptomyces sp. MWW064 for elucidating the rakicidin biosynthetic pathway

Position

δC

Relative enrichmentsa

[2-13C]acetate

[1-13C]-L-serine

1

169.2

0.89

1.58

2

54.9

1.14

1.19

3

72.5

1.57

1.07

4

172.7

1.31

1.95

5

167.6

0.68

6.61

6

52.5

0.77

1.15

7

36.5

1.00

1.00

8

166.0

0.77

1.43

9

118.8

3.46

1.54

10

138.4

1.03

13.97

11

137.9

0.95

0.67

12

117.1

1.02

1.33

13

172.5

1.61

1.26

14

41.7

1.86

1.36

15

78.1

1.25

1.21

16

33.9

1.91

1.39

17

32.8

1.30

1.55

18

27.0

2.64

1.26

19

28.9

1.15

1.58

20

31.3

3.37

1.43

21

22.1

1.05

1.28

22

14.0

3.37

1.61

23

15.4

1.51

1.07

24

13.3

1.75

1.32

  1. a 13C signal intensity of each peak in the labeled 1 divided by that of the corresponding signal in the unlabeled 1, respectively, normalized to give an enrichment ratio of 1 for the unenriched peak of C7. The numbers in bold type indicate 13C-enriched atoms from 13C-labeled precursors