Fig. 2

16S rRNA phylogenetic tree indicating the position of E. faecium LMG 8148 relative to other E. faecium strains and other enterococcal species (type strain = ^T). Lactobacillus plantarum was included as an outgroup. Genbank accession numbers of the aligned sequences are indicated between brackets. 16S rDNA sequences were aligned using MUSCLE, and the phylogenetic tree was determined using the neighbour-joining algorithm with the Kimura 2-parameter distance model in MEGA (version 7) [27]. A gamma distribution (shape parameter = 1) was used for rate variation among sites. The optimal tree with the sum of branch lengths = 0.1983 is shown, and nodes that appeared in more than 50 % of replicate trees in the bootstrap test (1000 replicates) are marked with their bootstrap support values