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Fig. 2 | Standards in Genomic Sciences

Fig. 2

From: The complete genome sequence of the methanogenic archaeon ISO4-H5 provides insights into the methylotrophic lifestyle of a ruminal representative of the Methanomassiliicoccales

Fig. 2

Phylogenetic analysis of Methanomassiliicoccales 16S rRNA gene sequences. Phylogenetic tree showing the relationships of methanogenic archaeon ISO4-H5 (shown in bold print) relative to other type and non-type strains within the order Methanomassiliicoccales. The phylogeny was inferred from 16S rRNA gene nucleotide sequences (1474 bp internal region) aligned using the Maximum Likelihood method based on the Kimura 2-parameter model [61]. Evolutionary analyses were conducted in MEGA6 [62]. The bootstrap consensus tree from 1000 replicates [63] was used to infer the evolutionary history of the taxa analysed. Bar: 0.05 substitutions per nucleotide position. The GenBank accession numbers of environmental sequences are displayed with the source habitat given in brackets. Strains whose genomes have been sequenced are marked with an asterisk. The initial tree for the heuristic search was obtained by applying the Neighbor-Joining method to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach. All positions containing gaps or missing data were eliminated, giving a total of 455 positions in the final dataset. The 16S rRNA gene sequence from Thermoplasma acidophilum 122-1B2 was used as an outgroup

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