Complete genome sequence of Leuconostoc gelidum subsp. gasicomitatum KG16-1, isolated from vacuum-packaged vegetable sausages

Table 6 The presence/absence of selected genes in L. gelidum strains

Gene name	LMG 18811^T	KG16-1	C120c	C122c	KSL4-2	PB1a	PB1e	PL111	LMG 22919^a	LMG 18297^T	JB7	Other Leuconostocs
Lacticin biosynthesis genes	-	+	+^b	-	-	-	-	-	-	-	-	-
Polyketide biosynthesis cluster	+	-	+	+	+	+	-	+	+^b,c	-	-	-
Pyruvate oxidase	+	-	+	+	+	+	+	+	+	+	+	Present in 15 other leuconostocs
Biofilm formation genes	+	-	+	+	+	+	+	+	+^b,c	+	+	L. mesenteroides KFRI-MG, L. mesenteroides subsp. mesenteroides J18, L. pseudomesenteroides LMG 11482^T
Collagen-binding protein	+	-	+	+	+	+	+	+	+^b,c	+	+	Present in all four L. pseudomesenteroides strains
Xylose-proton symporter	+	-	+	+	+	+	+	+	+^b,c	+	+	Present in 15 other leuconostocs
Mucus-binding protein	+	+^b	+	+	+	+	+	+	+^b,c	-	-	-
Accessory Sec system	+	+	+	+	+	+	+	+	+^b,c	-	+	L. fallax LMG 18975^a,b, L. pseudomesenteroides LMG 11482 ^T, L. kimchii C2 (partially)
Fused glutamate racemase/NTP pyrophosphatase	+	+	+	+	+	+	+	+	+^b,c	+	+	Encoded by two separate genes
Source of isolation	Broiler meat [2]	Vegetable sausages [4]	Vegetable salad [9]	Vegetable salad [13]	Fish/carrots [10]	Carrot [10]	Carrot [13]	Carrot [10]	Kimchi [65]	Kimchi [7]	Kimchi [6]

Strains LMG 18811^T, KG16-1, C120c, C122c, KSL4-2, PB1a, PB1e, PL111 and LMG 22919 belong to L. gelidum subsp. gasicomitatum; LMG 18297 ^T and JB7 – to L. gelidum subsp. gelidum. Strains with complete genomes are LMG 18811^T, KG16-1 and JB7; others have draft genomes. Gene names and locus_tags (either from KG16-1 or LMG 18811 ^T): lacticin biosynthesis genes lctA (LEKG_0458) and lctMT (LEKG_0461-0462); polyketide biosynthesis cluster (LEGAS_1827-1830); pyruvate oxidase poxB (LEGAS_1053); biofilm formation genes icaB and icaA (LEGAS_1065, LEGAS_1067); collagen-binding protein cna (LEGAS_1063); xylose-proton symporter xylT (LEGAS_1062); mucus-binding protein (LEGAS_0414); accessory Sec system genes secY2, asp1, asp2, asp3, secA2, nss, gtfA, gtfB, asp4 (LEKG_0540-0548); fused glutamate racemase/NTP pyrophosphatase (LEKG_0672)
^aPhylogenetic analysis (Figure S1 of Additional file 1) indicates that the genome, assigned to L. inhae LMG 22919, actually belongs to L. gelidum subsp. gasicomitatum species
^bGene is missing from the genome annotation
^cContains frameshift(s)

ISSN: 2524-6372