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Fig. 4 | Standards in Genomic Sciences

Fig. 4

From: Comparing polysaccharide decomposition between the type strains Gramella echinicola KMM 6050T (DSM 19838T) and Gramella portivictoriae UST040801-001T (DSM 23547T), and emended description of Gramella echinicola Nedashkovskaya et al. 2005 emend. Shahina et al. 2014 and Gramella portivictoriae Lau et al. 2005

Fig. 4

Polysaccharide hydrolysis by Gramella type strains G. echinicola DSM 19838T, G. portivictoriae DSM 23547T. Both strains were incubated in medium 514 (6 g/L carbon source mix) and HaHa (12 mg/L carbon source mix) for up to 14 days. G. echinicola DSM 19838T was incubated at 25 °C and G. portivictoriae DSM 23547T at 28 °C. Each 200 μL well of a microtiter plate was filled with a small portion of one of the AZO-CL-polysaccharides, −casein (Megazym, Bray, Ireland), charcoal-pectin, −gelatin in 100 μL medium. Each well was inoculated with 100 μL of a starved culture of the strains. The control wells were inoculated with 100 μL medium. The blue colour indicates the release of AZO- monomers and thus hydrolysis of the polysaccharide/peptide. A red-brown colour indicates growth of the strain (mixture of blue and yellow-orange). Black grains in the surrounding of the charcoal-pectin and -gelatine indicate hydrolysis

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Environmental Microbiome

ISSN: 2524-6372