Fig. 2

Electron micrograph of T. cyclicum ALM1 DSM 14477T. Micrographs of bacterial cells grown from a culture of DSM 14477T in DSMZ medium 925 at 28 °C were taken with a field-emission scanning electron microscope (FE-Merlin, Zeiss). Bacteria were fixed with formaldehyde and glutaraldehyde, washed with TRIS-EDTA buffer, dehydrated with a graded series of acetone, critical-point dried with liquid CO2, sputter coated with gold-palladium and imaged in a Zeiss Merlin with the HE-SE2 detector and inlens-detector in a 25:75 ratio with an acceleration voltage of 5 kV