Fig. 4

Schematic representation of the circular chromosome of M. panniformis FACHB1757. The scales indicate location in Mbp, starting with the initial coding region. Using Circos integrated the gene prediction results of COG function annotation, methylated modification, and some other information. From inner to outer circles: the first circle shows the GC skew (in purple and green), and the value is plotted as the deviation from the average GC skew of the entire chromosome sequence. The bars in the second circle (in black and red) represent the GC content, which is plotted using a 10-Kb sliding window. Positions of tRNA and rRNA are marked by green bars in the third circle. Bars in the fourth and fifth circle are colored according to COG function categories of CDS; the fourth is a backward strand and fifth is a forward strand. The sixth and seventh circles indicate m4C and m6A sites in CDS/rRNA/tRNA regions (in blue bars); the sixth circle is a backward strand and the seventh circle is a forward strand. In the eighth circle, red bars show the m4C and m6A sites in intergenic regions