Table 2 Project information

MIGS 31

Finishing quality

High-quality draft

MIGS-28

Libraries used

Two paired-end libraries of 180 bp, 380 bp and two mate-pair libraries of 2,000 bp, 6,000 bp, respectively

MIGS 29

Sequencing platforms

Illumina HiSeq 2000

MIGS 31.2

Fold coverage

~330× for GM4792 Lac⁺ and ~370× for GM4792 Lac^- (180 bp); ~100x (other libraries)

MIGS 30

Assemblers

ALLPATHS-LG Release 42411 [31]

MIGS 32

Gene calling method

RATT, Prodigal v2.5 [35]

Locus Tag

U068 for Lac⁺ and U069 for Lac^-

Genbank ID

CP011342 for Lac⁺ and CP011343 for Lac^-

GenBank Date of Release

Jun 6, 2015

GOLD ID

Gi0059689 for GM4792 Lac⁺ and Gi0059688 for GM4792 Lac^-

BIOPROJECT

PRJNA224130 for GM4792 Lac⁺ and PRJNA224131 for GM4792 Lac^-

SRA IDs

GM4792 Lac⁺ : SRR2596368, SRR2537294,

SRR2619692, SRR2619693

GM4792 Lac^- : SRR2529478, SRR1039666,

SRR2529494, SRR2533204

MIGS 13

Source Material Identifier

GM4792

Project relevance

Experimental evolution, Tree of Life