Fig. 1
Phylogenetic tree based on 16S rRNA gene sequences of Pyrinomonas methylaliphatogenes K22T (highlighted) and other cultivated strains and clonal phylotypes within the phylum Acidobacteria. Four of the acidobacterial subdivisions are included. The tree was constructed via a Bayesian inference model (MrBayes), using Markov Chain Monte Carlo (MCMC - 2,000,000 resamples, four chains, temperature = 0.5) sampling methods to calculate posterior distributions of trees in the ARB software environment. Posterior probability values ≥ 90 % are indicated by open circles, ≥80 % by filled circles, and ≥70 % by open diamonds. The scale bar represents a 0.1 change per nucleotide position. Strains whose genomes have been sequenced, are marked with an asterisk; G. fermentans H5T (NZ_AUAU00000000), H. foetida TMBS4T (AGSB00000000), C. thermophilum BT (CP002414), P. methylaliphatogenes K22T (CBXV000000000), Candidatus ‘S. usitatus’ Ellin6076 (CP000473), Candidatus ‘K. versatilis’ Ellin345 (CP000360), Acidobacterium capsulatum ATCC 51196T (CP001472), Edaphobacter aggregans Wbg-1T (JQKI00000000), Granulicella mallensis MP5ACTX9T (CP003130), Granulicella tundricola MP5ACTX9T (CP002480), Terriglobus roseus KBS63T (CP003379), and Terriglobus saanensis SP1PR4T (CP002467). The phylotypes strains used as an outgroup included Thermoanaerobaculum aquaticum MP-01T (JX4200244), Dictyoglomus thermophilum H-6-12T (X69194), Caldisericum exile AZM16c01T (AB428365), Hydrogenobacter hydrogenophilus Z-829T (Z30424), Thermodesulfobacterium thermophilum DSM 1276T (AF334601), Deinococcus roseus TDMA-uv51 (AB264136), Truepera radiovicrix RQ-24T (DQ022076), Thermus aquaticus YT-1 (L09663), and Thermus scotoductus SE-1T (AF032127)