Skip to main content
Fig. 2 | Standards in Genomic Sciences

Fig. 2

From: The complete genome, structural proteome, comparative genomics and phylogenetic analysis of a broad host lytic bacteriophage ϕD3 infecting pectinolytic Dickeya spp.

Fig. 2

SDS-PAGE and MS analysis of ϕD3 structural proteins. For SDS-PAGE electrophoresis ca. 109 pfu ml−1 were mixed with Laemmli buffer and frozen in liquid nitrogen for 1-2 min. following the boiling at 95 °C for 5 min. The phage proteins were separated in 12 % acrylamide SDS-PAGE gel for ca. 19 h t 50 V at 22 °C. The bands were stained with PageBlue Coomasie Blue (Thermo Scientific) according to protocol provided by the manufacturer. For MS analysis of phage structural proteins, protein bands obtained from SDS-PAGE were excised from gel with a sterile scalpel and sent to the mass spectrometry analysis to Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Science in Warsaw, Poland. Possible molecular functions of the unknown structural proteins were elucidated using Gene Sillico Protein Structure Prediction Meta-server [9]

Back to article page

Environmental Microbiome

ISSN: 2524-6372