Genome sequence of Anoxybacillus ayderensis AB04T isolated from the Ayder hot spring in Turkey

Table 6 List of several glycoside hydrolases (GHs) identified in various Anoxybacillus genomes

GH	Enzyme	Similarity within Anoxybacillus genome^a														Number of studied enzyme^b
GH	Enzyme	AB04^T	WK1	E13^T	SK3-4	DT3-1	TNO	G10	G2^T	AF/04^T	AK1	BCO1	KU2-6	Kn10	PS2	Number of studied enzyme^b
1	β-glucosidase	91.2	100	90.7	39.5	92.4	-	-	-	-	-	-	91.6	92.0	-	-
10	Endo-1,4-β-xylanase	100.00	-	-	-	-	-	-	-	-	-	-	-	-	-	-
13	α-amylase (cell-bound)	98.6	100.00	98.4	97.4	96.2	97.2	96.2	98.2	98.6	94.5	76.6	98.8	99.8	84.3	2 [16]
13	α-amylase (extracellular)	77.6	-		100.00	-	-	95.0	-	-	-	54.2	-	-	-	-
13	Pullulanase	93.4	100.00	93.9	90.5	89.5	95.6	89.4	91.9	93.2	88.9	59.9	94.8	98.0	67.4	1 [17]
13	Amylopullulanase (>200 kDa)	-	100.00	90.2	88.8	-	-	99.1	-	-	59.6	-	-	89.6	-	1 [18]
13	Amylopullulanase (<200 kDa)	-	-	-	-	-	-	-	-	-	-	100	-	-	-	-
13	CDase	95.8	100.00	95.6	92.5	92.0	94.7	92.3	95.1	94.9	93.6	96.6	95.9	98.1	78.8	1 [19]
13	Oligo-1,6-glucosidase	98.2	100.00	61.7	96.0	96.0	98.1	96.0	53.9	97.5	97.0	53.6	96.5	98.6	90.9	-
13	Trehalose-6-phosphate hydrolase	95.6	100.00	-	94.2	93.7	-	93.9	-	96.2	94.9	-	95.8	99.1	-	-
13	1,4-α-glucan branching enzyme	93.4	100.00	-	93.2	92.8	94.1	92.4	-	93.9		94.9	-	-	-	-
31	α-glucosidase	92.1	100.00	92.9	91.0	89.2	88.1	89.2	91.5	91.7	88.5	71.1	93.4	96.9	67.4	-
32	Sucrase-6-phosphate hydrolase	94.7	100.00	-	91.3	91.8	91.0	91.3	-	93.5	92.5	-	93.9	93.3	-	-
36	α-galactosidase	-	-	91.2	-	-	-	79.2	100	-	90.5	72.3	93.7	-	79.5	-
51	α-L-arabinofuranosidase	93.6	100.00	-	-	-	-	-	-	-	99.4	-	-	-	-	1 [22]
52	β-xylosidase	91.5	-	90.4	-	-	-	100	99.6	-		-	-	-	89.5	-
65	Sugar hydrolase/phosphorylase	-	100.00	-	94.9	94.1	-	94.0		-	94.1	-	-	96.6	-	-
67	α-glucuronidase	100.00	-	-	-	-	-	-	-	-	-	-	-	-	-	-

^aThe reference for the protein sequence alignment is denoted as 100 %; ^bThe numbers represent the respective cloned, purified, and characterized enzymes from Anoxybacillus species. AB04^T = A. ayderensis AB04^T [30]; WK1 = A. flavithermus WK1 [5, 29]; E13^T = A. flavithermus subsp. yunnanensis E13^T [35, 47, 48]; SK3-4 = Anoxybacillus sp. SK3-4 [45, 46]; DT3-1 = Anoxybacillus sp. DT3-1 [45, 46]; TNO = A. flavithermus TNO-09.006 [5, 44]; G10 = A. kamchatkensis G10 [40–42]; G2^T = A. gonensis G2^T [36]; AT^T = A. thermarum AF/04^T [33–35]; AK1 = A. flavithermus AK1 [5, 39]; BCO1 = Anoxybacillus sp. BCO1 [31, 32]; KU2-6 = Anoxybacillus sp. KU2-6(11); Kn10 = A. flavithermus Kn10 [5, 43]; PS2 = A. tepidamans PS2 [37]

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