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Table 3 Library information

From: Metagenomic analysis of planktonic microbial consortia from a non-tidal urban-impacted segment of James River

Label

Riffles_WGS

Riffles_16S

Sample label

Mayo South

Mayo South

Sample preparation method

Mobio Sterivexâ„¢ PowerWaterâ„¢ DNA Kit

Mobio Sterivexâ„¢ PowerWaterâ„¢ DNA Kit*

Library preparation

Ion Plus Fragment Library kit

16S fusion PCR [14]

Sequencing platform(s)

Ion Torrent PGM

Illumina MiSeq

Sequencing chemistry

PGM Sequencing 200 Kit (318 chip)

MiSeq Reagent Kit Version 3 (600 cycles PE)

Sequence size (GBp)

0.61

1.15

Number of reads

4.07 × 106

4.11 × 106

Mate pairs or paired ends

NA

empty

Sequencing library insert size

150–250

300

Average read length

133

292

Standard deviation for read length

43

0

  1. * DNA template from the same 3 L water sample was used for both the WGS and 16S libraries