Fig. 2
Phylogenetic tree of 16S RNA sequences highlighting the V. anguillarum NB10 position relative to other Vibrio and Beneckea strains. Type strains are indicated with a superscript "T". The strains and their corresponding GenBank accession numbers for 16S rRNA genes are: V. anguillarum M3, AY035897; V. anguillarum NB10 (chromosome 1, 233,624-235,167 bp), V. ordalii 2003/09/511-2063, AY530930; V. anguillarum ATCC 19264T, X16895; V. ordalii ATCC 33509, NR_044851; V. tapetis CECT 4600T, Y08430; V. splendidus LMG 4042T, AJ515230; V. tasmaniensis LMG 20012T, AJ316192; V. metschnikovii CIP 69.14T, X74711; B. parahaemolytica ATCC 17802T, AF388386; V. campbellii ATCC 25920T, X56575; B. alginolytica ATCC 17749T, X74690; V. coralliilyticus LMG 20984T, AJ440005; V. furnissii ATCC 35016T, X76336; V. vulnificus ATCC 27562T, X76333; V. cholerae CECT 514T, X76337; and V. mimicus ATCC 33653T, X74713. For outgroup strains, Grimonitia hollisae LMG 17719T, AJ514909; Enterovibrio coralii LMG 22228T, AJ842343; and Salinivibrio costicola ATCC 35508TT, X74699 were used. The tree uses sequences aligned by the RDP aligner, and uses the Jukes-Cantor corrected distance model to construct a distance matrix based on alignment model positions without the use of alignment inserts, and uses a minimum comparable position of 200. The tree is built with RDP Tree Builder, which uses Weighbor [52] with an alphabet size of 4 and length size of 1000. The building of the tree also involves a bootstrapping process repeated 100 times to generate a majority consensus tree [53]. Bar equals 1% sequence difference. Only significant bootstrap values are indicated