Fig. 2

Phylogenetic tree highlighting the position of Thermoanaerobacter thermohydrosulfuricus BSB-33 relative to other type strains within the Thermoanaerobacteraceae. The tree was inferred from aligned characters of the 16S rRNA sequences using maximum likelihood method within the software MEGA v5.1 (bootstrap: calculated with the Kimura 2-parameter model distance correction and 1000 replicates). The strains and their corresponding GenBank accession numbers for 16S rDNA respectively are: T. wiegelii Rt8.B1, NR_075059.1; T. thermohydrosulfuricus WC1, HM585213.1; T. thermohydrosulfuricus BSB-33, EU368841.2; T. thermohydrosulfuricus DSM567, L09161.1; T. siderophilus SR4, AF120479.1; T. mathranii DSM11426, NR_026397.1; T. italicus DSM9252, AJ250846.1; T. ethanolicus ATCC31550, L09162.1; T. brockii subsp. finii DSM3389, L09166.1; T. brockii subsp. brockii DSM1457, L09165.1; Thermoanaerobacter sp. X513, AF542520.1; Thermoanaerobacter sp. X514, AF542517.1; T. pseudethanolicus DSM2355, L09164; Thermoanaerobacter sp. X561, AF542518.1; T. tengcongensis MB4, AF209708.1; Clostridium thermocellum ATCC27405, L09173.1. Bootstrap values are indicated at nodes when larger than 60 %. Clostridium thermocellum was used as an out group. The branches are scaled in terms of the expected number of substitutions per site (scale bar)