Figure 1

Phylogenetic trees highlighting the position of Klebsiella sp. strain strain BRL6-2 relative to other type and non-type strains within the Gammaproteobacteria, based on (A) 16S ribosomal RNA phylogeny, and (B) whole genome classification based on pfams. Strains are shown with corresponding NCBI genome project ids listed within [11]. The 16S tree uses sequences aligned by the RDP aligner, the Jukes-Cantor corrected distance model to construct a distance matrix based on alignment model positions without the use of alignment inserts, and a minimum comparable position of 200. The tree is built with RDP Tree Builder, which uses Weighbor [12] with an alphabet size of 4 and length size of 1000. The building of the tree also involves a bootstrapping process repeated 100 times to generate a majority consensus tree [13]. The whole genome classification is a hierarchical clustering of pfams groups that was generated using the Integrated Microbial Genomes (IMG) system [14]. Succinimonas amylolytica DSM2873 , Succinatimonas hippei YIT12066, and Tolumonas auensis TA 4 DSM9187 are type strains with genomes available in IMG. All others are non-type strains.