Figure 4

Domain prediction (A) of the 89-kb region found in L. kunkeei EFB6 and its presence in other lactobacilli (B). A combined transmembrane topology and signal peptide predictor [41] was used to determine putative domains. The yellow blocks represent signal peptides, the white color of the arrows show the non-cytoplasmic part. Red blocks represent transmembrane regions and blue blocks predicted coiled-coil structures. To test whether this region exists in other L. kunkeei strains, we designed specific primer-pairs for each ORF (Table 5, Figure 4A). Predicted PCR product sizes are depicted in white boxes. The presence of the genes were tested for L. kunkeei EFB6, L. kunkeei HI3 (isolated from honey), L. kunkeei DSM 12361 (isolated from wine), and L. johnsonii DSM 10533 (isolated from human blood) (Figure 4B). The obtained PCR product sizes correlated with the predicted sizes (Table 5, Figure 4A). For L. johnsonii DSM 10533, no PCR product could be obtained.